Synthesis and comparative evaluation of cytotoxicity in vitro of new platinum complexes with 3-amino-α- tetralonespiro-5'-hydantoin

Cisplatin is one of the most successful compounds in the fight against cancer. Recently, interest was directed towards the development of cisplatin analogues which possess Nheterocyclic carrier ligands, coordinated to the cytotoxic platinum(II) moiety, instead of one or both of the am(m)ines. Hydantoins form a large group of derivatives widely applied in medicine and pharmacy, especially as anticonvulsants, antiarrhythmics, antibacterial drugs, cytotoxic agents etc. A new cis-[Pt(NH3)LCl2], where L is 3-amino-α-tetralonespiro-5'hydantoin was synthesized and studied. The molecular formula of the complex was confirmed by the elemental analysis, melting point and IR spectra. The results show that the coordination of the ligand with metal ion was realized by nitrogen atom of the amine group. On the basis of the results from the physicochemical investigation, the most probable molecular structure of the platinum complex was proposed. This compound as well as previously prepared and studied Pt(II) and Pt(IV) complexes with general formulae cis-[PtL2Cl2] and cis-[PtL2Cl4], where L is the same ligand 3-amino-α-tetralonespiro-5'hydantoin were investigated for cytotoxicity in vitro on HL-60 and SKW-3 human tumour cell lines. The results showed that all complexes exerted concentration dependent antiproliferative activity. Keywords—platinum complexes; N-heterocyclic ligands; cytotoxicicty